strain BP (3, 4) was used throughout this study
strain BP (3, 4) was used throughout this study. was expressed in under an inducible promoter. After purification by nickel-chelate affinity chromatography, the recombinant product was detected like a 47-kDa protein band in immunoblots with the anti-ScMp65 serum, as well as with CaMp65-specific monoclonal antibodies. Both ScMp65 and CaMp65 were assayed for antigenic activation in…