85, 79C87 [PubMed] [Google Scholar] 25
85, 79C87 [PubMed] [Google Scholar] 25. proteins. The processed supernatant fraction (25 l) was incubated with constitutively active ZIPK in Tos-PEG4-NH-Boc a 50-l total assay mixture containing 10 mm MgCl2, 1 g of ZIPK, 0.5 l of phosphatase inhibitor cocktail I Tos-PEG4-NH-Boc and II (Sigma), and 0.1 mm ATP for 5 min at 30 C.…