Two murine antibodies with specificity against the AP-tag (clone YC8 IgG2A/; clone EF10 IgG1/) had been selected and additional characterized
Two murine antibodies with specificity against the AP-tag (clone YC8 IgG2A/; clone EF10 IgG1/) had been selected and additional characterized. Biotinylation of cell surface area receptors Biotin ligase (BirA) was purified from transformed E. 0.5%/h). These outcomes suggest an driven internalization process actively. We also demonstrate advantages of particular biotinylation in comparison to traditional antibody…