Quickly, 96-well ELISA plates (Nalge Nunc International, Naperville, IL, USA) were coated with 100 l/well of DT, TT or PT antigen (16 Lf or 1 Lf or 06 g/ml, respectively) diluted in layer buffer (001 M sodium phosphate/014 M sodium chloride, pH 74 for TT and DT antigen or 005 M sodium carbonate, pH 96 for PT antigen) and incubated for 16C18 h in 37C
Quickly, 96-well ELISA plates (Nalge Nunc International, Naperville, IL, USA) were coated with 100 l/well of DT, TT or PT antigen (16 Lf or 1 Lf or 06 g/ml, respectively) diluted in layer buffer (001 M sodium phosphate/014 M sodium chloride, pH 74 for TT and DT antigen or 005 M sodium carbonate, pH 96…