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4. mice, the purified E2-Flag mutants elicited high-titer, Syncytial Virus Inhibitor-1 cross-reactive antibodies which were in a position to neutralize HCV infectious contaminants from two genotypes examined (1a and 2a). These results suggest that E1E2-Flag envelope glycoproteins could possibly be essential immunogen applicants for vaccine looking to stimulate broad HCV-neutralizing replies. Keywords:Hepatitis C pathogen, E1E2,…