To acquire recombinant protein for sortase response, aminoglycine pentapeptides (Gly5) were inserted simply by PCR in the N terminus of sfGFP, mCherry, and fHbp, and the fragments were cloned right into a pET-SUMO vector using the Champ pET SUMO Manifestation System (Life Systems) to include a His-SUMO-(Gly)5 label in the N terminus from the proteins appealing
To acquire recombinant protein for sortase response, aminoglycine pentapeptides (Gly5) were inserted simply by PCR in the N terminus of sfGFP, mCherry, and fHbp, and the fragments were cloned right into a pET-SUMO vector using the Champ pET SUMO Manifestation System (Life Systems) to include a His-SUMO-(Gly)5 label in the N terminus from the proteins…