Entire cell lysates were extracted utilizing a buffer containing 70% (v/v) 0
Entire cell lysates were extracted utilizing a buffer containing 70% (v/v) 0.5?M Tris (pH 6.8), 12.8% (w/v) SDS, 30% (v/v) glycerol, 6% (v/v) 2-mercapto-ethanol and 0.012% (w/v) bromophenol blue. the nuclear translocation of NFATc2, as dependant on immunofluorescence staining. NFAT reporter assays demonstrated that hypoxia-induced NFAT signaling activation would depend on HIF-2, however, not HIF-1.…