GFP-Cdk1 to GFP-Cdk6, GFP-cyclinA, GFP-cyclinB, GFP-cyclinC, and GFP-cyclinD were generated by inserting target genes into pEGFP-C3 vector (Clontech)
GFP-Cdk1 to GFP-Cdk6, GFP-cyclinA, GFP-cyclinB, GFP-cyclinC, and GFP-cyclinD were generated by inserting target genes into pEGFP-C3 vector (Clontech). counterstain at 1 mg/ml; LY-294002 hydrochloride, BrdU, and roscovitine had been bought from Sigma-Aldrich. The dual-luciferase reporter assay package was bought from Promega. Pets. A colony of vectors Schisanhenol were supplied by Dr. You Han (Xiamen College…