(B) Second round of amplification using the pre-amplified PCR products from (A) as new DNA templates to separately amplify exon 9 (using original primers, 216?bp) and exon 20 (using internal primers, 192?bp)
(B) Second round of amplification using the pre-amplified PCR products from (A) as new DNA templates to separately amplify exon 9 (using original primers, 216?bp) and exon 20 (using internal primers, 192?bp). cells from breast cancer patients primary and metastatic tissues, blood, and bone marrow. Single cells were screened for mutations in exons 9 and…